Construct: shRNA TRCN0000146547

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018289.2-1961s1c1
DNA Barcode:
CACTCAGTTCTGCGTTAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS53 (55275)
Gene Description:
VPS53 subunit of GARP complex
Transcript:
RefSeq NM_018289.2 (NON-CURRENT)
Match location:
Position 1961 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146547)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTCAGTTCTGCGTTAAATT
Hairpin Sequence:
5'-CCGG-CACTCAGTTCTGCGTTAAATT-CTCGAG-AATTTAACGCAGAACTGAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTCAGTTCTGCGTTAAATTCTCGAGAATTTAACGCAGAACTGAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTCAGTTCTGCGTTAAATTCTCGAGAATTTAACGCAGAACTGAGTG-3'

Other clones with same target sequence:

(none)