Construct: shRNA TRCN0000146552

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145241.1-277s1c1
DNA Barcode:
CAGAAGGTTTCGTTTAGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR31 (114987)
Gene Description:
WD repeat domain 31
Transcript:
RefSeq NM_145241.1 (NON-CURRENT)
Match location:
Position 277 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146552)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAAGGTTTCGTTTAGGTTT
Hairpin Sequence:
5'-CCGG-CAGAAGGTTTCGTTTAGGTTT-CTCGAG-AAACCTAAACGAAACCTTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAAGGTTTCGTTTAGGTTTCTCGAGAAACCTAAACGAAACCTTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAAGGTTTCGTTTAGGTTTCTCGAGAAACCTAAACGAAACCTTCTG-3'

Other clones with same target sequence:

(none)