Construct: shRNA TRCN0000146606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003906.3-5708s1c1
DNA Barcode:
CAGGAGCATTTACGGATTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCM3AP (8888)
Gene Description:
minichromosome maintenance complex component 3 associated protein
Transcript:
RefSeq NM_003906.3 (NON-CURRENT)
Match location:
Position 5708 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGCATTTACGGATTTAA
Hairpin Sequence:
5'-CCGG-CAGGAGCATTTACGGATTTAA-CTCGAG-TTAAATCCGTAAATGCTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGCATTTACGGATTTAACTCGAGTTAAATCCGTAAATGCTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGCATTTACGGATTTAACTCGAGTTAAATCCGTAAATGCTCCTG-3'

Other clones with same target sequence:

(none)