Construct: shRNA TRCN0000146607

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004227.3-3976s1c1
DNA Barcode:
CCCTGGTTCATATTTGAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYTH3 (9265)
Gene Description:
cytohesin 3
Transcript:
RefSeq NM_004227.3 (NON-CURRENT)
Match location:
Position 3976 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146607)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGTTCATATTTGAGTTT
Hairpin Sequence:
5'-CCGG-CCCTGGTTCATATTTGAGTTT-CTCGAG-AAACTCAAATATGAACCAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGTTCATATTTGAGTTTCTCGAGAAACTCAAATATGAACCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGTTCATATTTGAGTTTCTCGAGAAACTCAAATATGAACCAGGG-3'

Other clones with same target sequence:

(none)