Construct: shRNA TRCN0000146678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178540.2-805s1c1
DNA Barcode:
CTATTACTTCACCTACCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1QTNF9 (338872)
Gene Description:
C1q and TNF related 9
Transcript:
RefSeq NM_178540.2 (NON-CURRENT)
Match location:
Position 805 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATTACTTCACCTACCACAT
Hairpin Sequence:
5'-CCGG-CTATTACTTCACCTACCACAT-CTCGAG-ATGTGGTAGGTGAAGTAATAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATTACTTCACCTACCACATCTCGAGATGTGGTAGGTGAAGTAATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATTACTTCACCTACCACATCTCGAGATGTGGTAGGTGAAGTAATAG-3'

Other clones with same target sequence:

(none)