Construct: shRNA TRCN0000146805

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198851.1-949s1c1
DNA Barcode:
CGTGACAATGCATTTGATTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C22orf34 (348645)
Gene Description:
chromosome 22 open reading frame 34
Transcript:
RefSeq NM_198851.1 (NON-CURRENT)
Match location:
Position 949 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146805)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGACAATGCATTTGATTCA
Hairpin Sequence:
5'-CCGG-CGTGACAATGCATTTGATTCA-CTCGAG-TGAATCAAATGCATTGTCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGACAATGCATTTGATTCACTCGAGTGAATCAAATGCATTGTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGACAATGCATTTGATTCACTCGAGTGAATCAAATGCATTGTCACG-3'

Other clones with same target sequence:

(none)