Construct: shRNA TRCN0000146822

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033127.1-1947s1c1
DNA Barcode:
CAAATCCTTCATCCCTTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC16B (89866)
Gene Description:
SEC16 homolog B, endoplasmic reticulum export factor
Transcript:
RefSeq NM_033127.1 (NON-CURRENT)
Match location:
Position 1947 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146822)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATCCTTCATCCCTTCTTT
Hairpin Sequence:
5'-CCGG-CAAATCCTTCATCCCTTCTTT-CTCGAG-AAAGAAGGGATGAAGGATTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATCCTTCATCCCTTCTTTCTCGAGAAAGAAGGGATGAAGGATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATCCTTCATCCCTTCTTTCTCGAGAAAGAAGGGATGAAGGATTTG-3'

Other clones with same target sequence:

(none)