Construct: shRNA TRCN0000146900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153697.1-1126s1c1
DNA Barcode:
CTTTACATTTAGCTGCCCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD44 (91526)
Gene Description:
ankyrin repeat domain 44
Transcript:
RefSeq NM_153697.1 (NON-CURRENT)
Match location:
Position 1126 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTACATTTAGCTGCCCTAA
Hairpin Sequence:
5'-CCGG-CTTTACATTTAGCTGCCCTAA-CTCGAG-TTAGGGCAGCTAAATGTAAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTACATTTAGCTGCCCTAACTCGAGTTAGGGCAGCTAAATGTAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTACATTTAGCTGCCCTAACTCGAGTTAGGGCAGCTAAATGTAAAG-3'

Other clones with same target sequence:

(none)