Construct: shRNA TRCN0000146905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003787.1-813s1c1
DNA Barcode:
CCAGATGGAACAGATCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOL4 (8715)
Gene Description:
nucleolar protein 4
Transcript:
RefSeq NM_003787.1 (NON-CURRENT)
Match location:
Position 813 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGGAACAGATCATAAA
Hairpin Sequence:
5'-CCGG-CCAGATGGAACAGATCATAAA-CTCGAG-TTTATGATCTGTTCCATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGGAACAGATCATAAACTCGAGTTTATGATCTGTTCCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGGAACAGATCATAAACTCGAGTTTATGATCTGTTCCATCTGG-3'

Other clones with same target sequence:

(none)