Construct: shRNA TRCN0000146909

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013304.1-1134s1c1
DNA Barcode:
CATTTATCTCATGTGGCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZDHHC1 (29800)
Gene Description:
zinc finger DHHC-type containing 1
Transcript:
RefSeq NM_013304.1 (NON-CURRENT)
Match location:
Position 1134 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000146909)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTTATCTCATGTGGCACAA
Hairpin Sequence:
5'-CCGG-CATTTATCTCATGTGGCACAA-CTCGAG-TTGTGCCACATGAGATAAATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTTATCTCATGTGGCACAACTCGAGTTGTGCCACATGAGATAAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTTATCTCATGTGGCACAACTCGAGTTGTGCCACATGAGATAAATG-3'

Other clones with same target sequence:

(none)