Construct: shRNA TRCN0000147061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014765.1-2615s1c1
DNA Barcode:
CCCAGAAATGTAATTGCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TOMM20 (9804)
Gene Description:
translocase of outer mitochondrial membrane 20
Transcript:
RefSeq NM_014765.1 (NON-CURRENT)
Match location:
Position 2615 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAATGTAATTGCCAAA
Hairpin Sequence:
5'-CCGG-CCCAGAAATGTAATTGCCAAA-CTCGAG-TTTGGCAATTACATTTCTGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAATGTAATTGCCAAACTCGAGTTTGGCAATTACATTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAATGTAATTGCCAAACTCGAGTTTGGCAATTACATTTCTGGG-3'

Other clones with same target sequence:

(none)