Construct: shRNA TRCN0000147074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004389.1-744s1c1
DNA Barcode:
CAACCGAGATTATGTGTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CTNNA2 (1496)
Gene Description:
catenin alpha 2
Transcript:
RefSeq NM_004389.1 (NON-CURRENT)
Match location:
Position 744 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCGAGATTATGTGTTCAA
Hairpin Sequence:
5'-CCGG-CAACCGAGATTATGTGTTCAA-CTCGAG-TTGAACACATAATCTCGGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCGAGATTATGTGTTCAACTCGAGTTGAACACATAATCTCGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCGAGATTATGTGTTCAACTCGAGTTGAACACATAATCTCGGTTG-3'

Other clones with same target sequence:

(none)