Construct: shRNA TRCN0000147130

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012432.2-380s1c1
DNA Barcode:
CAGTGACTAATTGTGAGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SETDB1 (9869)
Gene Description:
SET domain bifurcated histone lysine methyltransferase 1
Transcript:
RefSeq NM_012432.2 (NON-CURRENT)
Match location:
Position 380 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147130)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTGACTAATTGTGAGTCTT
Hairpin Sequence:
5'-CCGG-CAGTGACTAATTGTGAGTCTT-CTCGAG-AAGACTCACAATTAGTCACTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTGACTAATTGTGAGTCTTCTCGAGAAGACTCACAATTAGTCACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTGACTAATTGTGAGTCTTCTCGAGAAGACTCACAATTAGTCACTG-3'

Other clones with same target sequence:

  1. TRCN0000285490