Construct: shRNA TRCN0000147136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017934.4-5059s1c1
DNA Barcode:
CCAGGAACCATTCAAGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHIP (55023)
Gene Description:
pleckstrin homology domain interacting protein
Transcript:
RefSeq NM_017934.4 (NON-CURRENT)
Match location:
Position 5059 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAACCATTCAAGTAAAT
Hairpin Sequence:
5'-CCGG-CCAGGAACCATTCAAGTAAAT-CTCGAG-ATTTACTTGAATGGTTCCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAACCATTCAAGTAAATCTCGAGATTTACTTGAATGGTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAACCATTCAAGTAAATCTCGAGATTTACTTGAATGGTTCCTGG-3'

Other clones with same target sequence:

(none)