Construct: shRNA TRCN0000147177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144718.2-4892s1c1
DNA Barcode:
CCCATTTGAAAGTCAGACATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPICE1 (152185)
Gene Description:
spindle and centriole associated protein 1
Transcript:
RefSeq NM_144718.2 (NON-CURRENT)
Match location:
Position 4892 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTTGAAAGTCAGACATA
Hairpin Sequence:
5'-CCGG-CCCATTTGAAAGTCAGACATA-CTCGAG-TATGTCTGACTTTCAAATGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTTGAAAGTCAGACATACTCGAGTATGTCTGACTTTCAAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTTGAAAGTCAGACATACTCGAGTATGTCTGACTTTCAAATGGG-3'

Other clones with same target sequence:

(none)