Construct: shRNA TRCN0000147243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013260.4-207s1c1
DNA Barcode:
GAAGAAGATGAGAACAGTAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAP30BP (29115)
Gene Description:
SAP30 binding protein
Transcript:
RefSeq NM_013260.4 (NON-CURRENT)
Match location:
Position 207 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAAGATGAGAACAGTAGA
Hairpin Sequence:
5'-CCGG-GAAGAAGATGAGAACAGTAGA-CTCGAG-TCTACTGTTCTCATCTTCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAAGATGAGAACAGTAGACTCGAGTCTACTGTTCTCATCTTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAAGATGAGAACAGTAGACTCGAGTCTACTGTTCTCATCTTCTTC-3'

Other clones with same target sequence:

(none)