Construct: shRNA TRCN0000147244

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014494.1-4312s1c1
DNA Barcode:
GCAAACATGGTGCTATTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNRC6A (27327)
Gene Description:
trinucleotide repeat containing adaptor 6A
Transcript:
RefSeq NM_014494.1 (NON-CURRENT)
Match location:
Position 4312 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147244)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAACATGGTGCTATTTCAA
Hairpin Sequence:
5'-CCGG-GCAAACATGGTGCTATTTCAA-CTCGAG-TTGAAATAGCACCATGTTTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAACATGGTGCTATTTCAACTCGAGTTGAAATAGCACCATGTTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAACATGGTGCTATTTCAACTCGAGTTGAAATAGCACCATGTTTGC-3'

Other clones with same target sequence:

(none)