Construct: shRNA TRCN0000147282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015490.3-1083s1c1
DNA Barcode:
GATCAGTTTGTACTCTGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC31B (25956)
Gene Description:
SEC31 homolog B, COPII coat complex component
Transcript:
RefSeq NM_015490.3 (NON-CURRENT)
Match location:
Position 1083 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGTTTGTACTCTGTGAT
Hairpin Sequence:
5'-CCGG-GATCAGTTTGTACTCTGTGAT-CTCGAG-ATCACAGAGTACAAACTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGTTTGTACTCTGTGATCTCGAGATCACAGAGTACAAACTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGTTTGTACTCTGTGATCTCGAGATCACAGAGTACAAACTGATC-3'

Other clones with same target sequence:

(none)