Construct: shRNA TRCN0000147293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032639.2-1115s1c1
DNA Barcode:
GTGGTTCCAGTATTAGACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEKHA8 (84725)
Gene Description:
pleckstrin homology domain containing A8
Transcript:
RefSeq NM_032639.2 (NON-CURRENT)
Match location:
Position 1115 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTTCCAGTATTAGACAAA
Hairpin Sequence:
5'-CCGG-GTGGTTCCAGTATTAGACAAA-CTCGAG-TTTGTCTAATACTGGAACCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTTCCAGTATTAGACAAACTCGAGTTTGTCTAATACTGGAACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTTCCAGTATTAGACAAACTCGAGTTTGTCTAATACTGGAACCAC-3'

Other clones with same target sequence:

(none)