Construct: shRNA TRCN0000147358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152378.1-1570s1c1
DNA Barcode:
GCGAATTTCTTTCAAAGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FCRLB (127943)
Gene Description:
Fc receptor like B
Transcript:
RefSeq NM_152378.1 (NON-CURRENT)
Match location:
Position 1570 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAATTTCTTTCAAAGCCAT
Hairpin Sequence:
5'-CCGG-GCGAATTTCTTTCAAAGCCAT-CTCGAG-ATGGCTTTGAAAGAAATTCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAATTTCTTTCAAAGCCATCTCGAGATGGCTTTGAAAGAAATTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAATTTCTTTCAAAGCCATCTCGAGATGGCTTTGAAAGAAATTCGC-3'

Other clones with same target sequence:

(none)