Construct: shRNA TRCN0000147374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007214.2-303s1c1
DNA Barcode:
GTGGTATCGTTTACGGTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC63 (11231)
Gene Description:
SEC63 homolog, protein translocation regulator
Transcript:
RefSeq NM_007214.2 (NON-CURRENT)
Match location:
Position 303 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGTATCGTTTACGGTTATT
Hairpin Sequence:
5'-CCGG-GTGGTATCGTTTACGGTTATT-CTCGAG-AATAACCGTAAACGATACCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGTATCGTTTACGGTTATTCTCGAGAATAACCGTAAACGATACCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGTATCGTTTACGGTTATTCTCGAGAATAACCGTAAACGATACCAC-3'

Other clones with same target sequence:

  1. TRCN0000292323