Construct: shRNA TRCN0000147390

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178230.1-224s1c1
DNA Barcode:
GTTCCTGCTTTCACAGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPIAL4A (164022)
Gene Description:
peptidylprolyl isomerase A (cyclophilin A)-like 4A
Transcript:
RefSeq NM_178230.1 (NON-CURRENT)
Match location:
Position 224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147390)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCTGCTTTCACAGAATTA
Hairpin Sequence:
5'-CCGG-GTTCCTGCTTTCACAGAATTA-CTCGAG-TAATTCTGTGAAAGCAGGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCTGCTTTCACAGAATTACTCGAGTAATTCTGTGAAAGCAGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCTGCTTTCACAGAATTACTCGAGTAATTCTGTGAAAGCAGGAAC-3'

Other clones with same target sequence:

  1. TRCN0000049232