Construct: shRNA TRCN0000147397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001031665.1-1437s1c1
DNA Barcode:
GTCAGAAATCATCCCTTCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF816 (125893)
Gene Description:
zinc finger protein 816
Transcript:
RefSeq NM_001031665.1 (NON-CURRENT)
Match location:
Position 1437 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGAAATCATCCCTTCAAT
Hairpin Sequence:
5'-CCGG-GTCAGAAATCATCCCTTCAAT-CTCGAG-ATTGAAGGGATGATTTCTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGAAATCATCCCTTCAATCTCGAGATTGAAGGGATGATTTCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGAAATCATCCCTTCAATCTCGAGATTGAAGGGATGATTTCTGAC-3'

Other clones with same target sequence:

(none)