Construct: shRNA TRCN0000147401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003970.1-4552s1c1
DNA Barcode:
GCTGATAGTTGATCACACATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYOM2 (9172)
Gene Description:
myomesin 2
Transcript:
RefSeq NM_003970.1 (NON-CURRENT)
Match location:
Position 4552 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATAGTTGATCACACATT
Hairpin Sequence:
5'-CCGG-GCTGATAGTTGATCACACATT-CTCGAG-AATGTGTGATCAACTATCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATAGTTGATCACACATTCTCGAGAATGTGTGATCAACTATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATAGTTGATCACACATTCTCGAGAATGTGTGATCAACTATCAGC-3'

Other clones with same target sequence:

(none)