Construct: shRNA TRCN0000147461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014803.2-2358s1c1
DNA Barcode:
CAGGAGTAACAACTGAGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF518A (9849)
Gene Description:
zinc finger protein 518A
Transcript:
RefSeq NM_014803.2 (NON-CURRENT)
Match location:
Position 2358 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAGTAACAACTGAGTTAA
Hairpin Sequence:
5'-CCGG-CAGGAGTAACAACTGAGTTAA-CTCGAG-TTAACTCAGTTGTTACTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAGTAACAACTGAGTTAACTCGAGTTAACTCAGTTGTTACTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAGTAACAACTGAGTTAACTCGAGTTAACTCAGTTGTTACTCCTG-3'

Other clones with same target sequence:

(none)