Construct: shRNA TRCN0000147543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018103.3-3089s1c1
DNA Barcode:
GAAGGCTGATAGAAGACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC8D (55144)
Gene Description:
leucine rich repeat containing 8 VRAC subunit D
Transcript:
RefSeq NM_018103.3 (NON-CURRENT)
Match location:
Position 3089 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGCTGATAGAAGACATAA
Hairpin Sequence:
5'-CCGG-GAAGGCTGATAGAAGACATAA-CTCGAG-TTATGTCTTCTATCAGCCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGCTGATAGAAGACATAACTCGAGTTATGTCTTCTATCAGCCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGCTGATAGAAGACATAACTCGAGTTATGTCTTCTATCAGCCTTC-3'

Other clones with same target sequence:

(none)