Construct: shRNA TRCN0000147583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030978.1-332s1c1
DNA Barcode:
GAAAGTGCTCACAAACTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARPC5L (81873)
Gene Description:
actin related protein 2/3 complex subunit 5 like
Transcript:
RefSeq NM_030978.1 (NON-CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAAGTGCTCACAAACTTCAA
Hairpin Sequence:
5'-CCGG-GAAAGTGCTCACAAACTTCAA-CTCGAG-TTGAAGTTTGTGAGCACTTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAAGTGCTCACAAACTTCAACTCGAGTTGAAGTTTGTGAGCACTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAAGTGCTCACAAACTTCAACTCGAGTTGAAGTTTGTGAGCACTTTC-3'

Other clones with same target sequence:

  1. TRCN0000312405