Construct: shRNA TRCN0000147602

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006024.4-2823s1c1
DNA Barcode:
GTTTCTGTTGACCTTGTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TAX1BP1 (8887)
Gene Description:
Tax1 binding protein 1
Transcript:
RefSeq NM_006024.4 (NON-CURRENT)
Match location:
Position 2823 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147602)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTCTGTTGACCTTGTTGAT
Hairpin Sequence:
5'-CCGG-GTTTCTGTTGACCTTGTTGAT-CTCGAG-ATCAACAAGGTCAACAGAAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTCTGTTGACCTTGTTGATCTCGAGATCAACAAGGTCAACAGAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTCTGTTGACCTTGTTGATCTCGAGATCAACAAGGTCAACAGAAAC-3'

Other clones with same target sequence:

(none)