Construct: shRNA TRCN0000147604

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006620.2-1636s1c1
DNA Barcode:
GCGATCTATTGACAAACCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HBS1L (10767)
Gene Description:
HBS1 like translational GTPase
Transcript:
RefSeq NM_006620.2 (NON-CURRENT)
Match location:
Position 1636 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147604)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATCTATTGACAAACCTTT
Hairpin Sequence:
5'-CCGG-GCGATCTATTGACAAACCTTT-CTCGAG-AAAGGTTTGTCAATAGATCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATCTATTGACAAACCTTTCTCGAGAAAGGTTTGTCAATAGATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATCTATTGACAAACCTTTCTCGAGAAAGGTTTGTCAATAGATCGC-3'

Other clones with same target sequence:

  1. TRCN0000353597