Construct: shRNA TRCN0000147682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001014372.1-383s1c1
DNA Barcode:
GACTATCAGCAGAAGTGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYT14P1 (401135)
Gene Description:
synaptotagmin 14 pseudogene 1
Transcript:
RefSeq NM_001014372.1 (NON-CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTATCAGCAGAAGTGATAA
Hairpin Sequence:
5'-CCGG-GACTATCAGCAGAAGTGATAA-CTCGAG-TTATCACTTCTGCTGATAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTATCAGCAGAAGTGATAACTCGAGTTATCACTTCTGCTGATAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTATCAGCAGAAGTGATAACTCGAGTTATCACTTCTGCTGATAGTC-3'

Other clones with same target sequence:

(none)