Construct: shRNA TRCN0000147706

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194314.1-1131s1c1
DNA Barcode:
GCAGTGTCCTAAATGTGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZBTB41 (360023)
Gene Description:
zinc finger and BTB domain containing 41
Transcript:
RefSeq NM_194314.1 (NON-CURRENT)
Match location:
Position 1131 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147706)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGTCCTAAATGTGATAA
Hairpin Sequence:
5'-CCGG-GCAGTGTCCTAAATGTGATAA-CTCGAG-TTATCACATTTAGGACACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGTCCTAAATGTGATAACTCGAGTTATCACATTTAGGACACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGTCCTAAATGTGATAACTCGAGTTATCACATTTAGGACACTGC-3'

Other clones with same target sequence:

(none)