Construct: shRNA TRCN0000147812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014670.2-222s1c1
DNA Barcode:
GCAGTAGCTAAGTTTCTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BZW1 (9689)
Gene Description:
basic leucine zipper and W2 domains 1
Transcript:
RefSeq NM_014670.2 (NON-CURRENT)
Match location:
Position 222 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTAGCTAAGTTTCTTGAT
Hairpin Sequence:
5'-CCGG-GCAGTAGCTAAGTTTCTTGAT-CTCGAG-ATCAAGAAACTTAGCTACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTAGCTAAGTTTCTTGATCTCGAGATCAAGAAACTTAGCTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTAGCTAAGTTTCTTGATCTCGAGATCAAGAAACTTAGCTACTGC-3'

Other clones with same target sequence:

(none)