Construct: shRNA TRCN0000147842

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005552.3-1289s1c1
DNA Barcode:
GCAGTTAAATAACTTGGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KLC1 (3831)
Gene Description:
kinesin light chain 1
Transcript:
RefSeq NM_005552.3 (NON-CURRENT)
Match location:
Position 1289 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147842)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTAAATAACTTGGCCTT
Hairpin Sequence:
5'-CCGG-GCAGTTAAATAACTTGGCCTT-CTCGAG-AAGGCCAAGTTATTTAACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTAAATAACTTGGCCTTCTCGAGAAGGCCAAGTTATTTAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTAAATAACTTGGCCTTCTCGAGAAGGCCAAGTTATTTAACTGC-3'

Other clones with same target sequence:

(none)