Construct: shRNA TRCN0000147905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017918.3-909s1c1
DNA Barcode:
GCTGTTAAGAGTAGGCAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCUB (55013)
Gene Description:
mitochondrial calcium uniporter dominant negative beta subunit
Transcript:
RefSeq NM_017918.3 (NON-CURRENT)
Match location:
Position 909 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTTAAGAGTAGGCAATTT
Hairpin Sequence:
5'-CCGG-GCTGTTAAGAGTAGGCAATTT-CTCGAG-AAATTGCCTACTCTTAACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTTAAGAGTAGGCAATTTCTCGAGAAATTGCCTACTCTTAACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTTAAGAGTAGGCAATTTCTCGAGAAATTGCCTACTCTTAACAGC-3'

Other clones with same target sequence:

(none)