Construct: shRNA TRCN0000147929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003165.1-3167s1c1
DNA Barcode:
GCAGAAACTAAAGCAGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STXBP1 (6812)
Gene Description:
syntaxin binding protein 1
Transcript:
RefSeq NM_003165.1 (NON-CURRENT)
Match location:
Position 3167 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAACTAAAGCAGCAAAT
Hairpin Sequence:
5'-CCGG-GCAGAAACTAAAGCAGCAAAT-CTCGAG-ATTTGCTGCTTTAGTTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAACTAAAGCAGCAAATCTCGAGATTTGCTGCTTTAGTTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAACTAAAGCAGCAAATCTCGAGATTTGCTGCTTTAGTTTCTGC-3'

Other clones with same target sequence:

(none)