Construct: shRNA TRCN0000147960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016475.2-387s1c1
DNA Barcode:
GCAGCTATTATCACCTTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JKAMP (51528)
Gene Description:
JNK1/MAPK8 associated membrane protein
Transcript:
RefSeq NM_016475.2 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000147960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTATTATCACCTTACTT
Hairpin Sequence:
5'-CCGG-GCAGCTATTATCACCTTACTT-CTCGAG-AAGTAAGGTGATAATAGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTATTATCACCTTACTTCTCGAGAAGTAAGGTGATAATAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTATTATCACCTTACTTCTCGAGAAGTAAGGTGATAATAGCTGC-3'

Other clones with same target sequence:

(none)