Construct: shRNA TRCN0000148038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032810.2-637s1c1
DNA Barcode:
GCATGTTACTTGGAGTGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATAD1 (84896)
Gene Description:
ATPase family AAA domain containing 1
Transcript:
RefSeq NM_032810.2 (NON-CURRENT)
Match location:
Position 637 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTTACTTGGAGTGATAT
Hairpin Sequence:
5'-CCGG-GCATGTTACTTGGAGTGATAT-CTCGAG-ATATCACTCCAAGTAACATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTTACTTGGAGTGATATCTCGAGATATCACTCCAAGTAACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTTACTTGGAGTGATATCTCGAGATATCACTCCAAGTAACATGC-3'

Other clones with same target sequence:

  1. TRCN0000101557
  2. TRCN0000308412