Construct: shRNA TRCN0000148053

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014837.3-2657s1c1
DNA Barcode:
GCCGTTTGAGAAATCCTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SMG7 (9887)
Gene Description:
SMG7 nonsense mediated mRNA decay factor
Transcript:
RefSeq NM_014837.3 (NON-CURRENT)
Match location:
Position 2657 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148053)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGTTTGAGAAATCCTTATT
Hairpin Sequence:
5'-CCGG-GCCGTTTGAGAAATCCTTATT-CTCGAG-AATAAGGATTTCTCAAACGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGTTTGAGAAATCCTTATTCTCGAGAATAAGGATTTCTCAAACGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGTTTGAGAAATCCTTATTCTCGAGAATAAGGATTTCTCAAACGGC-3'

Other clones with same target sequence:

  1. TRCN0000292208