Construct: shRNA TRCN0000148070

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153234.3-3093s1c1
DNA Barcode:
GCACCCATTCTGTAAATTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LIX1 (167410)
Gene Description:
limb and CNS expressed 1
Transcript:
RefSeq NM_153234.3 (NON-CURRENT)
Match location:
Position 3093 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148070)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCCATTCTGTAAATTGAA
Hairpin Sequence:
5'-CCGG-GCACCCATTCTGTAAATTGAA-CTCGAG-TTCAATTTACAGAATGGGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCCATTCTGTAAATTGAACTCGAGTTCAATTTACAGAATGGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCCATTCTGTAAATTGAACTCGAGTTCAATTTACAGAATGGGTGC-3'

Other clones with same target sequence:

(none)