Construct: shRNA TRCN0000148074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002474.1-1808s1c1
DNA Barcode:
GAGTTCTCCATCATCCATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYH11 (4629)
Gene Description:
myosin heavy chain 11
Transcript:
RefSeq NM_002474.1 (NON-CURRENT)
Match location:
Position 1808 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTTCTCCATCATCCATTAT
Hairpin Sequence:
5'-CCGG-GAGTTCTCCATCATCCATTAT-CTCGAG-ATAATGGATGATGGAGAACTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTTCTCCATCATCCATTATCTCGAGATAATGGATGATGGAGAACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTTCTCCATCATCCATTATCTCGAGATAATGGATGATGGAGAACTC-3'

Other clones with same target sequence:

(none)