Construct: shRNA TRCN0000148082

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020808.1-5510s1c1
DNA Barcode:
GCTTTGATGAAGTGTCTTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SIPA1L2 (57568)
Gene Description:
signal induced proliferation associated 1 like 2
Transcript:
RefSeq NM_020808.1 (NON-CURRENT)
Match location:
Position 5510 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148082)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGATGAAGTGTCTTGTA
Hairpin Sequence:
5'-CCGG-GCTTTGATGAAGTGTCTTGTA-CTCGAG-TACAAGACACTTCATCAAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGATGAAGTGTCTTGTACTCGAGTACAAGACACTTCATCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGATGAAGTGTCTTGTACTCGAGTACAAGACACTTCATCAAAGC-3'

Other clones with same target sequence:

(none)