Construct: shRNA TRCN0000148123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080821.1-556s1c1
DNA Barcode:
GCCCTTGATTGTCAGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM210B (116151)
Gene Description:
family with sequence similarity 210 member B
Transcript:
RefSeq NM_080821.1 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTGATTGTCAGATATTT
Hairpin Sequence:
5'-CCGG-GCCCTTGATTGTCAGATATTT-CTCGAG-AAATATCTGACAATCAAGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTGATTGTCAGATATTTCTCGAGAAATATCTGACAATCAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTGATTGTCAGATATTTCTCGAGAAATATCTGACAATCAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000319298