Construct: shRNA TRCN0000148267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018289.2-1185s1c1
DNA Barcode:
GATCAACCAAAGAAGCCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VPS53 (55275)
Gene Description:
VPS53 subunit of GARP complex
Transcript:
RefSeq NM_018289.2 (NON-CURRENT)
Match location:
Position 1185 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAACCAAAGAAGCCTAAA
Hairpin Sequence:
5'-CCGG-GATCAACCAAAGAAGCCTAAA-CTCGAG-TTTAGGCTTCTTTGGTTGATC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAACCAAAGAAGCCTAAACTCGAGTTTAGGCTTCTTTGGTTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAACCAAAGAAGCCTAAACTCGAGTTTAGGCTTCTTTGGTTGATC-3'

Other clones with same target sequence:

(none)