Construct: shRNA TRCN0000148285
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_014753.2-3657s1c1
- DNA Barcode:
- CCTCATGAAAGAAAGATCCTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMS1 (9790)
- Gene Description:
- BMS1 ribosome biogenesis factor
- Transcript:
- RefSeq NM_014753.2 (NON-CURRENT)
- Match location:
- Position 3657 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000148285)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCATGAAAGAAAGATCCTT
- Hairpin Sequence:
-
5'-CCGG-CCTCATGAAAGAAAGATCCTT-CTCGAG-AAGGATCTTTCTTTCATGAGG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCATGAAAGAAAGATCCTTCTCGAGAAGGATCTTTCTTTCATGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCATGAAAGAAAGATCCTTCTCGAGAAGGATCTTTCTTTCATGAGG-3'
Other clones with same target sequence:
-
TRCN0000278484
