Construct: shRNA TRCN0000148357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000850.3-1066s1c1
DNA Barcode:
CGTTCCTTTCTCCTGTTTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSTM4 (2948)
Gene Description:
glutathione S-transferase mu 4
Transcript:
RefSeq NM_000850.3 (NON-CURRENT)
Match location:
Position 1066 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCCTTTCTCCTGTTTATT
Hairpin Sequence:
5'-CCGG-CGTTCCTTTCTCCTGTTTATT-CTCGAG-AATAAACAGGAGAAAGGAACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCCTTTCTCCTGTTTATTCTCGAGAATAAACAGGAGAAAGGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCCTTTCTCCTGTTTATTCTCGAGAATAAACAGGAGAAAGGAACG-3'

Other clones with same target sequence:

  1. TRCN0000083698
  2. TRCN0000280824
  3. TRCN0000151859