Construct: shRNA TRCN0000148359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001012961.1-525s1c1
DNA Barcode:
CTATGTAACTATCCAGGCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1orf84 (149469)
Gene Description:
chromosome 1 open reading frame 84
Transcript:
RefSeq NM_001012961.1 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGTAACTATCCAGGCCTA
Hairpin Sequence:
5'-CCGG-CTATGTAACTATCCAGGCCTA-CTCGAG-TAGGCCTGGATAGTTACATAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGTAACTATCCAGGCCTACTCGAGTAGGCCTGGATAGTTACATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGTAACTATCCAGGCCTACTCGAGTAGGCCTGGATAGTTACATAG-3'

Other clones with same target sequence:

(none)