Construct: shRNA TRCN0000148452

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012135.1-1417s1c1
DNA Barcode:
CCACAGTGACTGAGTTTGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM50B (26240)
Gene Description:
family with sequence similarity 50 member B
Transcript:
RefSeq NM_012135.1 (NON-CURRENT)
Match location:
Position 1417 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148452)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGTGACTGAGTTTGATT
Hairpin Sequence:
5'-CCGG-CCACAGTGACTGAGTTTGATT-CTCGAG-AATCAAACTCAGTCACTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGTGACTGAGTTTGATTCTCGAGAATCAAACTCAGTCACTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGTGACTGAGTTTGATTCTCGAGAATCAAACTCAGTCACTGTGG-3'

Other clones with same target sequence:

(none)