Construct: shRNA TRCN0000148500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173678.1-1656s1c1
DNA Barcode:
CGTGGAGATCAATGGAATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCAF2 (285966)
Gene Description:
TRPM8 channel associated factor 2
Transcript:
RefSeq NM_173678.1 (NON-CURRENT)
Match location:
Position 1656 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGAGATCAATGGAATCAA
Hairpin Sequence:
5'-CCGG-CGTGGAGATCAATGGAATCAA-CTCGAG-TTGATTCCATTGATCTCCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGAGATCAATGGAATCAACTCGAGTTGATTCCATTGATCTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGAGATCAATGGAATCAACTCGAGTTGATTCCATTGATCTCCACG-3'

Other clones with same target sequence:

(none)