Construct: shRNA TRCN0000148542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002780.2-1026s1c1
DNA Barcode:
CGAGAAATGAAACAGGACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSG4 (5672)
Gene Description:
pregnancy specific beta-1-glycoprotein 4
Transcript:
RefSeq NM_002780.2 (NON-CURRENT)
Match location:
Position 1026 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGAAATGAAACAGGACCTT
Hairpin Sequence:
5'-CCGG-CGAGAAATGAAACAGGACCTT-CTCGAG-AAGGTCCTGTTTCATTTCTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGAAATGAAACAGGACCTTCTCGAGAAGGTCCTGTTTCATTTCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGAAATGAAACAGGACCTTCTCGAGAAGGTCCTGTTTCATTTCTCG-3'

Other clones with same target sequence:

(none)