Construct: shRNA TRCN0000148569

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024556.1-432s1c1
DNA Barcode:
CCAGTAATGTTCGATCCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM118B (79607)
Gene Description:
family with sequence similarity 118 member B
Transcript:
RefSeq NM_024556.1 (NON-CURRENT)
Match location:
Position 432 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000148569)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAATGTTCGATCCACAT
Hairpin Sequence:
5'-CCGG-CCAGTAATGTTCGATCCACAT-CTCGAG-ATGTGGATCGAACATTACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAATGTTCGATCCACATCTCGAGATGTGGATCGAACATTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAATGTTCGATCCACATCTCGAGATGTGGATCGAACATTACTGG-3'

Other clones with same target sequence:

(none)